Articles techniques et présentations

Array-based technologies are important for many applications in drug discovery, microbiology and cell biology. A large-scale array of single cells allows high-throughput monitoring of behaviors of individual cells in parallel, avoiding the lack of cell specificity inherent to bulk measurement methods. Here, we designed a passive-pumping microfluidic device for trapping cells in an array and used the COMSOL Multiphysics® software to simulate the velocity field of the laminar flow within the device. Our results show that 93.5% of the traps were occupied by cells and 71.0% of all the traps captured only a single cell. The single cell trapping microfluidic device developed here can be useful in experiments requiring monitoring of single cells, due to its high efficiency and the ease of operation.